Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: 8D5]

Specifications

Product Data
Clone Name	8D5
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Blot: 1/500 - 1/2000. Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	KLH conjugated synthetic peptide corresponding to amino acids (CEDLAGFKELFQTPG) of Human KI67
Specificity	Recognizes KI67
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	358 kDa
Gene Name	marker of proliferation Ki-67
Database Link	Entrez Gene 4288 Human UniProt ID P46013
Background	Ki67, also known as MKI67, it is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Synonyms	Ki67 antigen, MKI67, Proliferation marker
Reference Data

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