CD11b Rat Monoclonal Antibody [Clone ID: 3A33]

Specifications

Product Data
Clone Name	3A33
Applications	FC
Recommend Dilution	Flow Cytometry: ? 0.3 µg/106 cells (Ref.1).
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Peritoneal macrophages from C57 B1/6 x DBA/2 F1 hybrid mice. (Ref.1)
Specificity	This antibody is specific to Alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer.
Isotype	IgG2a
Formulation	PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent. Label: APC-Cy7 State: Liquid purified Ig fraction. Label: Allophycocyanin-Cyanine 7
Concentration	lot specific
Conjugation	APC-Cy7
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Database Link	UniProt ID P05555
Background	CD11b (Integrin alpha M subunit) combines with CD18 (Integrin beta2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). (Ref.2-6) Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. (Ref.2,7-10) In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process. (Ref.4,11) Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. (Ref.12)
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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