Cd86 Rat Monoclonal Antibody [Clone ID: GL1]

Specifications

Product Data
Clone Name	GL1
Applications	FC
Recommend Dilution	Flow Cytometry: < / = 0.1 µg/10e6 cells. (Ref.1-7)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	LPS-activated mouse B cells. (Ref.1)
Specificity	This antibody recognises CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4.
Isotype	IgG2a
Formulation	PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent. Label: PE-Cy7 State: Liquid purified Ig fraction. Label: R-Phycoerythrin-Cyanine 7
Concentration	lot specific
Conjugation	PE-Cy7
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	CD86 antigen
Database Link	Entrez Gene 12524 Mouse UniProt ID P42082
Background	CD86 (also known as B7-2) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. (Ref.1-5) CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation. (Ref.1,5) CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. (Ref.1-9). The monoclonal antibody GL1 blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells. (Ref.1,3,5)
Synonyms	CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1
Reference Data

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