T Cell Receptor / TCR gamma/delta Mouse Monoclonal Antibody [Clone ID: TCR1]

Specifications

Product Data
Clone Name	TCR1
Applications	FC, FN, IHC, IP
Recommend Dilution	Flow Cytometry. (Ref.1-3) Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections). (Ref.5) Immunoprecipitation. (Ref.1-3) In ovo depletion of gamma delta T cells. (Ref.6,7)
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Immunogen	Outbred chicken thymocytes and Ig-negative blood lymphocytes.
Specificity	This antibody recognizes TCR gamma/delta. It precipitates a heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50-kDa upon reduction) on Chicken peripheral blood T cells. (Ref.1,2) Deglycosylation of the heterodimer yields two polypeptides of Mr 35-kDa and 32-kDa from TCR1 precipitates.
Isotype	IgG1
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. Unlike human and mouse, in which gamma/delta cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of gamma/delta T cells. The frequency of TCR gamma/delta cells is usually 20-25% of the total blood T cells, but may reach approximately 50% in chickens of 6 months of age. The majority of TCRgamma/delta+ cells in the thymus and blood are CD4-CD8-, although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRgamma/delta+ cells migrate into the spleen and intestine, most of them begin to express CD8. Although the biological function of gamma/delta T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ gamma/delta T cells may also be involved in down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reactions.
Synonyms	T-Cell Receptor delta, T-Cell Receptor gamma, T-Cell Receptor gamma delta, TCRD, TCRG, TCR gamma, TCR delta
Reference Data

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