Nuclei Mouse Monoclonal Antibody [Clone ID: 235-1]

Specifications

Product Data
Clone Name	235-1
Applications	IF, IHC, IP
Recommend Dilution	Immunoprecipitation. Immunofluorescence: 1/100. Immunohistochemitry: 1/100. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. Also works for Immunohistochemistry on paraformaldehyde fixed cryostat sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human cell homogenate.
Specificity	This antibody is an excellent Marker for Human cells in xenographic model research. It reacts specifically with Human cells, including neurons and embryonic stem cells, with no reactivity to Rat or Mouse cells. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. By Immunoprecipitation the antibody reacts with an 80 kDa and 70 kDa band.
Isotype	IgG1
Formulation	PBS State: Supernatant State: Liquid Culture Supernatant.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Reference Data

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