KLH Mouse Monoclonal Antibody [Clone ID: 12B4.G3.A8]

Specifications

Product Data
Clone Name	12B4.G3.A8
Applications	ELISA, IHC, WB
Recommend Dilution	This anti-KLH antibody can be used as a Control in various assays or in ELISA to detect KLH or KLH conjugates. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000.
Host	Mouse
Clonality	Monoclonal
Immunogen	A BALB/c mouse was immunized with KLH (Keyhole Limpet Hemocyanin) followed by hybridoma development.
Specificity	This antibody reacts specifically with KLH.
Isotype	IgG1
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Purified State: Liquid (sterile filtered) purified Ig fraction Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
Synonyms	Keyhole Limpet Hemocyanine
Reference Data

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