HMG1 (HMGB1) Mouse Monoclonal Antibody [Clone ID: J2E1]

Specifications

Product Data
Clone Name	J2E1
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western blot (1:500 - 1:2,000), recommended starting dilution is 1:1,000. Immunohistochemistry (1:100 - 1:300), recommended starting dilution is 1:200.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human HMGB1 (1-215aa) purified from Baculovirus
Specificity	The antibody recognizes human HMGB1. Other species not tested.
Isotype	IgG2b, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	high mobility group box 1
Database Link	NP_002119 Entrez Gene 3146 Human UniProt ID P09429
Background	High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.
Synonyms	Sulfoglucuronyl carbohydrate binding protein, SBP-1, HMGB1, HMG3, HMG1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Base excision repair

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