Ribonuclease Inhibitor (RNH1) Mouse Monoclonal Antibody [Clone ID: AT1H23]

Specifications

Product Data
Clone Name	AT1H23
Applications	ELISA, ICC/IF, IHC, WB
Recommend Dilution	ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (5 µg/ml). This RNH1 antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Ribonuclease inhibitor 1(RNH1) (7-461aa) purified from E. coli
Specificity	The antibody recognizes Human and Mouse RNH1 (7-461). Other species not tested.
Isotype	IgG2a, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	ribonuclease/angiogenin inhibitor 1
Database Link	NP_002930 Entrez Gene 107702 MouseEntrez Gene 6050 Human UniProt ID P13489
Background	Ribonuclease inhibitor 1 (RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50 kDa. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both intracellular and extracellular RNases in the cytosol. RNH1 is a role in abolishing the ribonucleolytic and angiogenic activities of angiogenin.
Synonyms	ribonuclease/angiogenin inhibitor 1, ribonuclease/angiogenin inhibitor, RAI
Reference Data

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