HCV Core protein Mouse Monoclonal Antibody [Clone ID: A1/3D1]

Specifications

Product Data
Clone Name	A1/3D1
Applications	ELISA
Recommend Dilution	ELISA: HCV Core Protein antibody (clone A1/3D1) was tested using indirect ELISA method. The testing plates were coated with HCV non-structural protein (NS), Capsid Protein (C) and the mixture of NS and C separately. The neat culture supernatant of hybridoma showed a strong reaction with HCV capsid protein (OD492 value > 2.0) and mixture of NS and C (OD492 value >1.5), and have no reaction with HCV Non-structural protein (OD492 value < 0.03). Not suitable for use in Western Blots. Has been used successfully in the recognition of in-vitro translated HCV core protein.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptides derived from HCV Capsid Protein
Specificity	HCV Core Protein antibody (clone A1/3D1) is reactive with Recombinant Capsid Protein C (CPC)+Envelope Protein M (EPM) (core) (1a.a. -142a.a.) and Synthetic CPC (1a.a. -61a.a.). This clone recognizes different antigenic determinants of HCV capsid protein. Cross-Reactivity: It showed no cross reaction with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).
Isotype	IgG1
Formulation	0.01M PBS, pH 7.0 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml
Purification	Affinity chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Background	The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.
Synonyms	Hepatitis C Virus core protein
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.