Trichosanthin Mouse Monoclonal Antibody [Clone ID: TCST1]

Specifications

Product Data
Clone Name	TCST1
Applications	ELISA
Recommend Dilution	ELISA.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly purified Trichosanthin / TCST.
Specificity	Reactive with Trichosanthin (TCST).
Isotype	IgG1
Formulation	0.01M PBS, pH 7.2 without preservatives. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Background	Trichosanthin, an abortifacient, immunosuppressive and anti-tumor protein purified from the traditional Chinese herb medicine Tian Hua Fen, is a potent inhibitor against HIV-1 replication. Under normal enzymatic digestion conditions, trichosanthin cleaves the supercoiled double-stranded DNA to produce nicked circular and linear DNA. Trichosanthin has no effect on linear double-stranded DNA. Neither does it convert relaxed circular duplex DNA into a supercoiled form in the presence of ATP. Thus trichosanthin is not a DNA gyrase. However, trichosanthin can cleave the relaxed circular DNA into a linear form, indicating that both the circular as well as the supercoiled forms are essential for trichosanthin recognition. In addition, trichosanthin contains one calcium metal ion per protein molecule, which presumably is related to its endonucleolytic activity.
Synonyms	TCST, rRNA N-glycosidase
Reference Data

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