Lime1 (86-202) Mouse Monoclonal Antibody [Clone ID: mLIME-05]

Specifications

Product Data
Clone Name	mLIME-05
Applications	IP, WB
Recommend Dilution	Immunoprecipitation (use murine splenic T cells as positive control). Western blot: 1-5 μg/ml (reducing conditions).
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant protein corresponding to amino acids 86-202 of mouse LIME
Specificity	This antibody reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.
Isotype	IgG2a
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	Lck interacting transmembrane adaptor 1
Database Link	Entrez Gene 72699 Mouse UniProt ID Q9EQR5
Background	LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Synonyms	LIME1, LIME, LIME-1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1
Reference Data

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