Human Lambda Light Chain Mouse Monoclonal Antibody [Clone ID: 4C2]

Specifications

Product Data
Clone Name	4C2
Applications	ELISA, FC, IHC
Recommend Dilution	Flow cytometry: 2-8 µg/ml. Positive control: RAMOS human cell line. Immunohistochemistry on frozen and paraffin sections. ELISA.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody reacts with lambda light chains (22.5 kDa) of human immunoglobulin.
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Background	Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Reference Data

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