Vinculin (VCL) Mouse Monoclonal Antibody [Clone ID: VIN-54]

Specifications

Product Data
Clone Name	VIN-54
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1 - 2 μg/ml. Immunohistochemistry on frozen sections: 2 - 4 μg/ml.
Reactivity	Chicken, Human, Mouse, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human vinculin, purified from uterus
Specificity	This antibody reacts to Vinculin.
Isotype	IgG1
Formulation	1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative. State: Purified State: Lyphilized purified Ig fraction
Reconstitution Method	Restore with 1.2% sodium acetate or neutral PBS.
Concentration	0,1 mg/ml (after reconstitution with PBS)
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at -20°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	vinculin
Database Link	Entrez Gene 22330 MouseEntrez Gene 305679 RatEntrez Gene 7414 Human UniProt ID P18206
Background	Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane . both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95%). Vinculinis mapped to 10q22.1-q23.
Synonyms	Metavinculin
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton

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