ICAM3 Mouse Monoclonal Antibody [Clone ID: MEM-04]

Specifications

Product Data
Clone Name	MEM-04
Applications	FC, FN, IHC
Recommend Dilution	Flow cytometry: 5 µg/ml. Immunohistochemistry on paraffin sections: 10 µg/ml. Positive control: tonsil. Functional application: blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Leukocytes of a patient suffering from LGL-type leukemia
Specificity	The antibody MEM-04 reacts with CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).
Isotype	IgG1
Formulation	Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. State: Low Endotoxin State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	intercellular adhesion molecule 3
Database Link	Entrez Gene 3385 Human UniProt ID P32942
Background	CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
Synonyms	ICAM-3, ICAM-R, CDw50
Reference Data

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