Amyloid beta (1-42 specific) Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	ELISA: dilution 1:4000. This biotin conjugated antibody can detect Aβ42 in Sandwich ELISA assay. For sandwich ELISA use clone NT 4A2, Cat.-No AM26042PU-N as capture antibody. Immunoblot.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	KLH conjugated to a short peptide with amino acid sequence corresponding to the C-terminal of Aβ42
Specificity	This antibody recognizes the C-terminal sequence (MVGGVVIA) of Aβ42 and full length Aβ42. The antibody does not cross react with amyloid beta peptide 40 in dot blotting and ELISA. Cross-reactivity to amyloid beta peptide 43 is less than 1% in ELISA.
Isotype	IgG1
Formulation	0.01M PBS, pH 7.0 ± 0.1 Label: Biotin State: Liquid purified IgG fraction Stabilizer: 1% Gelatin Preservative: 0.1% Proclin-300
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Biotin
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Amyloid beta peptide 42 (Aβ42) is best known for its role in the formation of senile plaques in the brain of patients with Alzheimer’s disease. Aβ42 and Aβ40 are the two major amyloid peptides that are produced after cleavage of amyloid precursor protein by secretases. Aβ42 (42 amino acids) is very fibrillogenic. The beta pleated structure of Aβ42 constituents the initial and key component of the insoluble amyloid fibril in senile plaque. It is widely accepted that Aβ42 contributes to the pathogenesis of Alzheimer’s disease. One proposition is that the deposition of amyloid fibril onto the brain tissue results in Alzheimer’s disease. Another is that the neurotoxicity of Aβ42 oligomer is the cause of the disease.
Reference Data

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