6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: 16-002]

Specifications

Product Data
Clone Name	16-002
Applications	ELISA, WB
Recommend Dilution	ELISA.  Western Blot: 1/500-1/1000 (Reduced conditions).
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified, recombinant C-terminal His6-tagged protein expressed in E. coli
Specificity	This antibody detects the C-terminal His6-tag, probably recognizes N-terminal His6-tag
Isotype	IgG2b
Formulation	PBS pH 7.4 State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C to -70°C. Avoid repeated freezing and thawing.
Background	6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
Synonyms	6xHis-Tag, His Tag, HIS6 Tag, HHHHHH Tag
Reference Data

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