Activated Macrophages Rat Monoclonal Antibody [Clone ID: 88a]

Specifications

Product Data
Clone Name	88a
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on frozen sections: 2 μg/ml (1:100). Has been described to work in FACS. Suggested positive control: Mouse spleen.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Supernatant of stimulated lymphocytes
Specificity	This antibody detects Macrophages, dendritic cells, granulocytes.
Isotype	IgG2a
Formulation	Stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative. State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	Reconstitute by adding 0.5ml distilled water.
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	The antigen is a 200kD (glyco-)protein with unknown function. The antigen is expressed at low levels by bone marrow monocytes and granulocytes, and increases upon maturation in vitro. The expression is also enhanced after MIF treatment of macrophages. The antigen is displayed by both immature and mature Kupffer cells.
Note	Antigen distribution: Isolated cells: The antigen is found on granulocytes, monocytes and bone marrow cells. Tissue sections: The 88a antigen is found on dendritic cells in T- and B-cell areas as well as on marginal zone macrophages in lymphoid tissues. Typical macrophages in other organs show less or no expression of the 88a antigen.
Reference Data

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