ECs2973 Mouse Monoclonal Antibody [Clone ID: 13C4]

Specifications

Product Data
Clone Name	13C4
Applications	ELISA, FN, WB
Recommend Dilution	Western blot: 1/10 as starting dilution. Fuctional Assay: Inhibition of the biological activity of the toxin.
Reactivity	Escherichia coli
Host	Mouse
Clonality	Monoclonal
Specificity	The monoclonal antibody 13C4 recognizes the 1B subunit of Shiga-like toxin 1.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Database Link	UniProt ID P69179
Background	Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share >99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct.The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.
Synonyms	sltB, Verocytotoxin 1 subunit B, Verotoxin 1 subunit B
Reference Data

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