Sftpd Mouse Monoclonal Antibody [Clone ID: IVG8]

Specifications

Product Data
Clone Name	IVG8
Applications	ELISA, IHC, WB
Recommend Dilution	Western blot. Immunoassay (detection antibody for ELISA on wells coated with human SP-D). The typical starting working dilution is 1:10. Immunohistochemistry on paraffin sections of rat lung. It cannot be used for staining of paraffin sections of human lung.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Specificity	The monoclonal antibody IVG8 recognizes surfactant protein D (SP-D).
Formulation	PBS State: Purified State: Liquid purified 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	surfactant protein D
Database Link	Entrez Gene 25350 Rat UniProt ID P35248
Background	SP-D belongs to the collectin familiy. These proteins are oligomeric proteins composed of carbohydrate-recognition domains (CRD) attached to collagenous regions. They are structurally similar to the ficolins although they make use of different CRD structures: C-type lectin domain for the collectins. The anti-microbial effector mechanisms of SP-D are direct opsonization, neutralization, and agglutination. Thus limiting the infection and concurrently orchestrating the subsequent adaptive immune response. The lung is the major site of synthesis of SP-D, where the molecules are produced and secreted onto the epithelial surface by alveolar type II cells and unciliated bronchial epithelial cells. SP-D is also found in different epithelial cells of the gastrointeststinal tract and in epithelial cells of exocrine glands. SP-D synthesis and secretion increase significantly after inflammatory stress. Increased amounts of SP-D in lavage and tissue, particularly in type II pneumocytes, in Clara cells and in hyperplastic goblet cells are found in inflamed lungs. The localization of SP-D in endocytic vesicles and in lysosomal granules of alveolar macrophages suggests that a receptor-mediated uptake occurs. SP-D binds to apoptotic neutrophils and enhances their clearance by alveolar macrophages.
Synonyms	Lung surfactant protein D, SP-D, PSP-D, PSPD, SFTP4, Collectin-7, COLEC7
Reference Data

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