beta 2 Adrenergic Receptor (ADRB2) Mouse Monoclonal Antibody [Clone ID: 6H8]

Specifications

Product Data
Clone Name	6H8
Applications	ELISA, FN
Recommend Dilution	Flow Cytometry: A431 Human epidermoid cells in suspension for 1 h at 4°C in PBS containing monoclonal Antibody 6H8 (260 nM). After 1 h of incubation, the cells were fixed with 2% Formaldehyde (Ref.1: Lebesgue). The typical starting working dilution is 1/50. Functional Assays: Antibody 6H8 functions as an agonist in neonatal Rat cardiomyocytes (Ref 1, Lebesgue). Furthermore, Fab fragments of agonist-like antibody 6H8 behave as antagonist (Ref.2: Mijares). Immunoassay: Useful as detector in an ELISA setting (Ref.1: Lebesgue). Positive Control: Human epidermoid carcinoma cell A431 line.
Reactivity	Guinea Pig, Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Free peptide Beta2-H19C
Specificity	The monoclonal antibody 6H8 recognizes Human beta-2-Adrenoceptor.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	adrenoceptor beta 2
Database Link	Entrez Gene 154 Human UniProt ID P07550
Background	The b -adrenoceptors can be divided into b1, b2, b3 and b4-adrenoceptors defined in terms of agonist potencies, b2-adrenoceptors displayed a higher selectivity for nor-adrenaline than for adrenaline. B2-receptors are mainly postsynaptic and are located on a number of tissues including blood vessels, bronchi, GIT, skeletal muscle, liver and mast cell. Activation results in vasodilatation, bronchodilation, relaxation of the GIT, glycogenolysis in the liver, tremor in skeletal muscle and inhibition of histamine release from mast cells. Transduction is via G-proteins coupled to the intracellular second messenger adenylate cyclase. B-receptors are positively coupled to adenylate cyclase via activation of Gs G-protein, however activation of the b2-adrenoceptors results in stimulation and inhibition of adenylate cyclase. The b2-receptor selective agonists are widely used in the treatment of asthma and other related bronchospastic conditions. They are commonly used in the treatment of angina pectoris, cardiac arrhythmia and for the long-term treatment of patients who survive myocardial infarction. B-receptor antagonists have also been used as anti-hypertensive for a number of years. Beta -blockers have also proven useful in the treatment of conditions such as migraine, anxiety disorders, hyperthyroidism, alcohol withdrawal and when applied topically are useful in the treatment of glaucoma and ocular hypertension.
Synonyms	ADRB2, ADRB2R, B2AR, Beta-2 adrenoreceptor
Reference Data

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