Vcam1 Rat Monoclonal Antibody [Clone ID: 6C7.1]

Specifications

Product Data
Clone Name	6C7.1
Applications	FC, FN, IF, IHC, IP
Recommend Dilution	Immunohistochemistry on frozen sections. Flow cytometry: The typical starting working dilution is 1:50. Functional assays. Immunoflourescence: The typical starting working dilution is 1:50. Immunoprecipitation.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse endothelial cells
Specificity	The monoclonal antibody 6C7.1 recognizes mouse vascular cell adhesion molecule (VCAM-1).
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	vascular cell adhesion molecule 1
Database Link	Entrez Gene 22329 Mouse UniProt ID P29533
Background	VCAM-1 (~ 81 kDa) is a member of a subclass of the immunoglobulin superfamily (IgSF). IgSF members are ligands for integrins. Cell adhesion molecules (CAMs) have important roles in the immune response, immune surveillance and cell-cell recognition, especially in leukocyte-endothelial cell adhesion. CAMs on the surface of leukocytes and endothelial cells are actively involved in the recruitment of specific leukocyte subsets into different tissues. VCAM-1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Cell adhesion molecules, like VCAM-1, are upregulated on cerebral vessels during inflammatory conditions of the central nervous system such as experimental autoimmune encephalomyelitis (EAE), a model system for multiple sclerosis. Administration of monoclonal antibody 6C7.1 has been shown to inhibit or diminish clinical or pathological signs of EAE. VCAM-1 is a receptor for encephalomyocarditis virus on murine vascular endothelial cells. Expression of VCAM-1 on vascular endothelial cells is induced by TNF-alpha, IL-1, IFN-gamma or endotoxin. VCAM-1 is a ligand for the integrins alpha4beta1 (VLA-4) and alpha4beta7 (LPAM-1). These integrins are constitutively expressed by thymocytes, lymphocytes and monocytes. VCAM-1/VLA-4 interaction may play a pathophysiological role in immune responses and as well as in leukocyte emigration to sites of inflammation.
Synonyms	V-CAM 1, INCAM-100, L1CAM, VCAM-1
Reference Data

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