Mbl1 Rat Monoclonal Antibody [Clone ID: 8G6]

Specifications

Product Data
Clone Name	8G6
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Immunohistochemistry on frozen sections (2,6): Tissue sections were fixed in 4% PBS-buffered formaldehyde and pretreated with 2% hydrogen peroxide in methanol for 20 minutes at 4⁰C to quench endogenous peroxidases. Primary antibody 8g6, 2µg/ml. As negative control rat IgG2a was used (Ref.2). The typical starting working dilution is 1:50. Immunoassays (2). Immunoflourescence (3,4). Western blot (1): A non-reduced sample treatment was used. The band sizes are 191, 263 and 316 kDa (Ref.1). The typical starting working dilution is 1:50.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Purified mouse MBL-A
Specificity	This antibody detects MBL-A.
Isotype	IgG
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Gene Name	mannose-binding lectin (protein A) 1
Database Link	Entrez Gene 17194 Mouse UniProt ID P39039
Background	Mannose Binding Lectin (MBL), also called mannosebinding protein (MBP), is a calcium dependent oligomeric protein that belongs to the collectin family of proteins. It contains a collagen-like domain and a carbohydrate recognition domain enabling MBL to recognize carbohydrates (such as mannose and N-acetylglucosamine) on pathogens. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways, by using attached mannose binding lectin-associated serine proteases (MASP-2) in an antibody- and C1q-independent manner. MASP-2 permits cleavage of C4 and C2 to form a C3 convertase. Once it has bound, MBL exhibits complement-dependent antibacterial activities such as microbial opsonization and/or microbial lysis via membrane attack complexes and therefore plays an important role in innate immunity. In human, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, termed MBL-A and MBL-C. The MBL-A concentration in serum is about 6-fold lower compared to that of MBL-C... MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. Moreover, it has been shown that MBL-A deficient mice have aberrant antigen-specific IgM responses and suffer from increased susceptibility to infection.
Synonyms	MBL-A; MBP-A; Mbpa; Mlb1; S-MBP
Note	Note that the monoclonal antibody 8G6 is a calcium-dependent antibody.
Reference Data

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