CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: 3D3]

Specifications

Product Data
Clone Name	3D3
Applications	FC
Recommend Dilution	Flow cytometry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified glycosylated recombinant human FcgammaRIIa2
Specificity	This antobody recognizes CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).
Formulation	Phosphate buffered saline (PBS) State: Purified State: Liquid Ig fraction Preservative: 15 mM sodium azide, approx. pH 7.4
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	Fc fragment of IgG receptor IIa
Database Link	Entrez Gene 2212 Human UniProt ID P12318
Background	CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).
Synonyms	Fc-gamma RII-a, Fc-gamma-RIIa, CDw32, FCGR2A, FCG2, FCGR2A1, IGFR2
Reference Data

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