RPP20 (POP7) Mouse Monoclonal Antibody [Clone ID: 1F11]

Specifications

Product Data
Clone Name	1F11
Applications	ELISA, WB
Recommend Dilution	ELISA. Immunoblotting. Recommended Solvent: 100 mM PBS or Tris-HCl, pH 7.0
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant GST-hRPP20 (NCBI accession number AAC24113, expression vector pGEC-2T), expressed in E.coli.
Specificity	Specificity has been tested in ELISA. In immunoblotting, under less stringent washing conditions, a weaker band of approximately 60 kDa is also detected (Figure 1, Right Panel). Additional tests for cross reactivity have not yet been performed.
Isotype	IgG2a
Formulation	10 mM Ammonium Bicarbonate buffer State: Purified State: Lyophilized purified Ig fraction Stabilizer: 2 mg BSA
Reconstitution Method	Dissolve in 200 μl buffer for a 0.5 mg/ml antibody concentration in 1% BSA
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C or Add up to 0.05% Sodium Azide for long term storage.
Predicted Protein Size	20 kDa
Gene Name	POP7 homolog, ribonuclease P/MRP subunit
Database Link	Entrez Gene 10248 Human UniProt ID O75817
Background	The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle-specific proteins. On average, snoRNPs contain about 6-10 protein subunits.
Synonyms	hPOP7, RPP20
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.