MRP1 (ABCC1) Rat Monoclonal Antibody [Clone ID: MRPr1]

Specifications

Product Data
Clone Name	MRPr1
Applications	IF, IHC, WB
Recommend Dilution	Flow Cytometry: Use at least 1/20-1/50. Cell permeabilization required with 10% lyzing solution followed by primairy antibody and anti-Rat-FITC.  Western blotting: Use at least 1/20-1/50. Use an anti-Rat HRP.  Immunocytochemistry: Use at least 1/20-1/50 on Aceton fixed cell preparation.  Immunohistochemstry on Acetone-Fixed Frozen Sections: at least 1/20-1/50.  Immunohistochemstry on Formalin-Fixed Paraffin Embedded Tissues: 1/20.  Pretreatment (0.01M Citrate, 10 min. at 100°C) may increase the performance of this antibody.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Bacterial fusion protein of MRP, containing a segment of 168 amino acids (aa 192-360) in the amino-proximal half of the protein.
Specificity	This Monoclonal antibody MRPr1 reacts with an epitope of MRP, a 180-195 kD transmembrane transporter protein overexpressed in various Human non-P-glycoprotein MDR tumor cell lines.  It does not cross-react with the Human MDR1 and MDR3 gene products (See Flens et al. 1994).
Formulation	State: Supernatant State: Serum-Free Tissue Culture Supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for 3 months or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ATP binding cassette subfamily C member 1
Database Link	Entrez Gene 4363 Human UniProt ID P33527
Background	MRP1 (also known as ABCC, GS-X, ABC29) functions as a multispecific organic anion transporter, with (oxidized) glutathione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. It also transports drugs and other hydrophobic compounds in presence of glutathione. MRP1 protein is found predominantly in the heart, lung, small intestine, brain, and skin.
Synonyms	Multidrug resistance-associated protein 1, LTC4 transporter
Reference Data

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