Synaptophysin (SYP) Rabbit Monoclonal Antibody [Clone ID: MRQ-40]

Specifications

Product Data
Clone Name	MRQ-40
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections.  Recommended Dilution: 1/100-1/500.  Positive Control: Pancreas.  Staining pattern: Cytoplasmic.  Preparation and Pretreatment 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.  2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) technique in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Specificity	This Antibody reacts with neuroendocrine cells of Human adrenal medulla, carotid body, skin, pituitary, thyroid, lung, pancreas and gastrointestinal mucosa. Positive staining is seen in neurons of the brain, spinal cord, retina, and Paneth’s cells in the gastrointestinal tract and gastric parietal cells. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. The expression of synaptophysin is independent of the presence of NSE or other neuroendocrine markers. Anti-Synaptophysin is an independent broad-range marker of neural and neuroendocrine differentiation.
Formulation	Tris Buffer State: Liquid Supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	synaptophysin
Database Link	Entrez Gene 6855 Human UniProt ID P08247
Background	Synaptophysin is a synaptic vesicle glycoprotein with 4 transmembrane domain MW 38 kDa. This protein is present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is also marker of endocrine tumors.
Synonyms	Neuroendocrine Marker
Reference Data

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