Cadherin 16 (CDH16) Mouse Monoclonal Antibody [Clone ID: MRQ-33]

Specifications

Product Data
Clone Name	MRQ-33
Applications	IHC
Recommend Dilution	Immunohistochemistry on frozen and paraffin sections: 1/100 - 1/500. Instructions for use: 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive control: Kidney, chromophobe renal cell carcinoma Staining pattern: Cytoplasmic, membranous
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody reacts to KSP-Cadherin.
Isotype	IgG1
Formulation	PBS, pH 7.4 State: Ascites State: Liquid Ascites Stabilizer: 0,9% BSA Preservative: 0,09% sodium azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	cadherin 16
Database Link	Entrez Gene 1014 Human UniProt ID O75309
Background	Kidney-specific cadherin (Ksp-Cadherin) is a novel, kidney-specific member of the cadherin family of cell adhesion molecules. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Different cadherins, including E-cadherin, cadherin-6, and Ncadherin, have been investigated in renal cell cancers, demonstrating possible correlations of tumor dedifferentiation and the presence of lymph node metastasis with loss of cadherins. Mazal et al. investigated the utility of using Ksp-Cadherin to distinguish chromophobe renal cell carcinoma from oncocytoma. He found a membranous pattern of staining in 96% of 30 chromophobe carcinomas, and in only 6% of 31 oncocytomas leading them to conclude that this is a useful antibody in differentiating these two lesions. Shen et al., on the other hand, found Ksp-Cadherin positivity in 100% of 13 chromophobe RCCs, and 95% of 20 oncocytomas.
Synonyms	CDH16, Kidney-specific cadherin, KSP-Cadherin
Reference Data

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