CD1 (CD1A) Mouse Monoclonal Antibody [Clone ID: O10+C1A/711]

Specifications

Product Data
Clone Name	O10+C1A/711
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein. Western Blotting: 0.5-1 μg/ml. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human thymus cells (O10); Recombinant Human CD1a protein (C1A/711).
Specificity	Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. Anti-CD1a is reported to be a new marker for perivascular epithelial cell tumor (PEComa). Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	49 kDa
Gene Name	CD1a molecule
Database Link	Entrez Gene 909 Human UniProt ID P06126
Background	At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin.
Synonyms	T6/Leu-6, hTa1
Reference Data

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