CD6 Mouse Monoclonal Antibody [Clone ID: C6/372 + 3F7B5]

Specifications

Product Data
Clone Name	C6/372 + 3F7B5
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 0.5-1 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein. Western Blotting: 0.5-1 µg/ml. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: CCRF-CEM, Jurkat cells, Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant CD6 protein (C6/372) and Human rheumatoid synovial T cell line ST-1 (3F7B5).
Specificity	CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular “scavenger receptor cysteine-rich” (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease. Cellular Localization: Cell surface and cytoplasmic.
Isotype	IgG1
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	90-130 kDa
Gene Name	CD6 molecule
Database Link	Entrez Gene 923 Human UniProt ID P30203
Synonyms	T12, TP120
Reference Data

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