BUB3 Mouse Monoclonal Antibody [Clone ID: AT2H6]

Specifications

Product Data
Clone Name	AT2H6
Applications	ELISA, WB
Recommend Dilution	The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human BUB3 (1-328aa) purified from E. coli
Isotype	IgG2b, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	BUB3, mitotic checkpoint protein
Database Link	NP_004716 Entrez Gene 9184 Human UniProt ID O43684
Background	BUB3 (budding uninhibited by benzimidazoles 3 homolog) is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments.
Synonyms	Mitotic checkpoint protein BuB3, BuB3L, hBuB3
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cell cycle

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.