Pcdhga1 (808-931, Cytopl. Dom.) Mouse Monoclonal Antibody [Clone ID: S159-5]

Specifications

Product Data
Clone Name	S159-5
Applications	IF, WB
Recommend Dilution	Western blot: 1/1000, 1 μg/ml was sufficient for detection of PCDHG (pan) in 20 μg of Rat brain lysate by colorimetric immunoblot analysis using HRP conjugated secondary antibody. Immunocytochemistry.
Reactivity	Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A-subfamily: amino acids ~807-930, B-subfamily: amino acids ~789-912 and C subfamily).
Specificity	This antibody detects a ~100 kDa protein. Cross-reacts with all Gamma- protocadherin-A, -B and -C proteins.
Isotype	IgG1
Formulation	PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	protocadherin gamma subfamily A, 1
Database Link	Entrez Gene 93709 Mouse UniProt ID Q91XZ0
Background	The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
Synonyms	Protocadherin gamma, PCDH gamma, Gamma protocadherin
Reference Data

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