ERK1 / ERK2 pTyr204 Rabbit Polyclonal Antibody

CAT#: AP01886PU-N

ERK1 / ERK2 pTyr204 rabbit polyclonal antibody, Aff - Purified

Size: 50 ug 100 ug 25 ul



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CNY 1999.00

CNY 4437.00


货期*
2周

规格
    • 100 ug

Cited in 5 publications.

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Specifications

Product Data
Applications IF, IHC, WB
Recommend Dilution Western Blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Immunofluorescence: 1/50-1/200.
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Tyrosine 204.
Specificity This antibody detects endogenous levels of ERK1/2 protein when phosphorylated at Tyr204.
Formulation Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration 1.0 mg/ml
Purification Affinity Chromatography using epitope-specific immunogen.
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Predicted Protein Size ~ 44,42 kDa
Background The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
Synonyms ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (5)

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