ERK1 / ERK2 pTyr204 Rabbit Polyclonal Antibody
CAT#: AP01886PU-N
ERK1 / ERK2 pTyr204 rabbit polyclonal antibody, Aff - Purified
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CNY 1999.00
CNY 4437.00
货期*
2周
规格
Cited in 5 publications. |
Specifications
Product Data | |
Applications | IF, IHC, WB |
Recommend Dilution | Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200. Immunofluorescence: 1/50-1/200. |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Tyrosine 204. |
Specificity | This antibody detects endogenous levels of ERK1/2 protein when phosphorylated at Tyr204. |
Formulation | Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). |
Concentration | 1.0 mg/ml |
Purification | Affinity Chromatography using epitope-specific immunogen. |
Conjugation | Unconjugated |
Storage Condition | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Predicted Protein Size | ~ 44,42 kDa |
Background | The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. |
Synonyms | ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK |
Reference Data |
Citations (5)
The use of this Antibodies has been cited in the following citations: |
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piR-121380 Is Involved in Cryo-Capacitation and Regulates Post-Thawed Boar Sperm Quality Through Phosphorylation of ERK2 via Targeting PTPN7
,null,
Frontiers in Cell and Developmental Biology
,PubMed ID 35155446
|
Oncogene-dependent addiction to carbohydrate-responsive element binding protein in hepatocellular carcinoma
,null,
Cell Cycle
,PubMed ID 29965794
|
ROLE OF METHIONINE ADENOSYLTRANSFERASE α2 AND β PHOSPHORYLATION AND STABILIZATION IN HUMAN HEPATIC STELLATE CELL TRANS-DIFFERENTIATION
,null,
Journal of cellular physiology
,PubMed ID 25294683
|
Facilitatory effects of fetuin-A on atherosclerosis
,null,
Atherosclerosis
,PubMed ID 26828753
|
HMMR Maintains the Stemness and Tumorigenicity of Glioblastoma Stem-like Cells
,null,
Cancer research
,PubMed ID 24710409
|
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