Caspase 10 (CASP10) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin sections: 1/50-1/200. Western blot: 1/500-1/1000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 410-462 of Human Caspase 10.
Specificity	This antibody detects endogenous levels of Caspase 10 protein (region surrounding Lys442).
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 59 kDa
Gene Name	caspase 10
Database Link	Entrez Gene 843 Human UniProt ID Q92851
Background	Four isoforms of caspase 10 (caspase 10a, 10b, 10c, and 10d) having the same prodomain but different mature large and small subdomain, have been described. Caspase 10 contains two death domains (DED) involved in linking to the death effector domain of the adapter protein FADD and recruiting the complex to TNFR1 and Fas. The inactive procaspase 10 is variably expressed in many tissues and cell lines as a cytosolic protein. The mature form of caspase 10 comprises two subunits, p23/p17 (splice isoforms) and p12. Interestingly, a caspase 9- dependent processing of caspase 10 by caspase 6 in cell-free extracts has recently been suggested. Caspase 10 can cleave and activate caspases 3, 4, 6, 7, 8, and 9. This is followed by cleavage of numerous key proteins, including the nuclear protein PARP.
Synonyms	CASP-10, CASP10, MCH4, FLICE2
Reference Data

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