B Raf (BRAF) Rabbit Polyclonal Antibody

CAT#: AP06500PU-N

B Raf (BRAF) rabbit polyclonal antibody, Aff - Purified



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CNY 4,640.00


货期*
2周

规格
    • 100 ug

Product images

Specifications

Product Data
Applications IHC, WB
Recommend Dilution Western Blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide, corresponding to amino acids 560-610 of Human Raf-B.
Specificity This antibody detects endogenous levels of B-Raf protein.
(region surrounding Ala597)
Formulation Phosphate buffered saline (PBS), pH 7.2.
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Preservative: 0.05% Sodium Azide
Concentration 1.0 mg/ml
Purification Affinity Chromatography using epitope-specific immunogen
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Predicted Protein Size ~86 kDa
Gene Name B-Raf proto-oncogene, serine/threonine kinase
Background Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.
Synonyms BRAF, BRAF1, RAFB1, p94
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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