TMS1 (PYCARD) Rabbit Polyclonal Antibody

CAT#: AP06792PU-N

TMS1 (PYCARD) rabbit polyclonal antibody, Aff - Purified



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CNY 4,640.00


货期*
2周

规格
    • 100 ug

Cited in 1 publication.

Product images

Specifications

Product Data
Applications IF, IHC, WB
Recommend Dilution
Western Blot: 1/500-1/1000.
Immunofluorescence: 1/50-1/200.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide, corresponding to the N-terminual of Human TMS1.
Specificity This antibody detects endogenous levels of ASC protein. (region surrounding Glu13)
Formulation PBS, pH~7.2
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE); 50% glycerol
Preservative: 0.02% Sodium Azide
Concentration 1.0 mg/ml
Purification Affinity Chromatography using epitope-specific immunogen
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Predicted Protein Size ~22 kDa
Gene Name PYD and CARD domain containing
Background Caspase-associated recruitment domains (CARDs) mediate the interaction between adaptor proteins such as APAF1 and the proform of caspases (e.g. CASP9) participating in apoptosis. ASC (apoptosis-associated speck-like protein containing a CARD, also known as TMS1or PYCARD) is a member of the CARD-containing adaptor protein family. ASC is a 195 amino acid protein that contains an N-terminal pyrin-like domain (PYD) and an 87 residue C-terminal CARD. This motif is characteristic of numerous proteins involved in apoptotic signaling. Fluorescence microscopy demonstrates a ring-like expression in some transfected cells. Immunofluorescence microscopy demonstrates that induction of apoptosis causes a CARD-dependent shift from diffuse cytoplasmic expression to punctate or spherical perinuclear aggregates. Western blot analysis shows expression of 22 kDa ASC in leukemia and melanoma cell lines. ASC exhibits intriguing behavior by forming an aggregate and appearing as a speck during apoptosis induced by retinoic acid and other anti-tumor drugs. The ASC gene maps to human chromosome 16p12-p11.2.
Synonyms TMS1
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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