Synaptotagmin 1 (SYT1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Western blot: 1/500~1/1000. Immunofluorescence: 1/100~1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized non-phosphopeptide derived from human Synaptotagmin I/II around the phosphorylation site of Threonine 202/199 (R-K-Tp-L-N).
Specificity	This antibody detects endogenous levels of total Synaptotagmin I/II protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	synaptotagmin 1
Database Link	Entrez Gene 20979 MouseEntrez Gene 25716 RatEntrez Gene 6857 Human UniProt ID P21579
Background	Synaptotagmin 1 is a 60kDa member of the synaptotagmin family, expressed in membranes of synaptic vesicles in rostral, phylogenetically younger brain regions and in some endocrine tissues. Ca2+ is bound by synaptogamin whose C2 domains then bind phospholipids, trafficking vesicles to the synaptic membrane and exocytosis. This results in fast synchronous neurotransmitter release. Synaptotagmin also binds neurexins, syntaxin and AP2 independently of Ca2+ binding. Phosphorylation of synaptotagmin by protein kinases is thought to modulate its activity. Synaptotagmin 2 belongs to the synaptotagmin family of multi-domained, integral membrane proteins of synaptic vesicles which are thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
Synonyms	Synaptotagmin I/II, SYT1/SYT2
Reference Data
Protein Families	Secreted Protein, Transmembrane

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