Luciferase (Photinus pyralis) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA: 1/200-1/1000. Immunofluorescence: 20 µg/ml. Western Blot: 1/1000-1/5000. Immunohistochemistry: 1/1,000-1/5,000. Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, immunocytochemistry, and most immunological methods requiring high titer and specificity.
Host	Goat
Clonality	Polyclonal
Immunogen	Luciferase Photinus pyralis (Firefly)
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Isotype	IgG
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Purified State: Liquid (sterile filtered) purified IgG fraction Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Concentration	lot specific
Purification	Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P08659
Background	Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Reference Data

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