WEE1 pSer123 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	ELISA: 1/1,000. Immunohistochemistry: 1/50-1/100. Dot Blot: 1/500.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S123 of human WEE1
Specificity	This antibody detects WEE1 pSer123.
Isotype	Ig
Formulation	PBS with 0.09% (W/V) Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	WEE1 G2 checkpoint kinase
Database Link	Entrez Gene 7465 Human UniProt ID P30291
Background	This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Synonyms	Wee1A kinase, WEE1hu
Note	Molecular weight: 71597 Da
Reference Data

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