c-Myb (MYB) (557-569) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.5-1.5 µg/ml. Detects a band of Approx 85 kDa in lysates of cell line Jurkat.
Reactivity	Bovine, Canine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide corresponding to internal region of Human c-Myb
Specificity	This antibody is expected to recognize all reported isofoms (NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1; NP_001155132.1).
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	MYB proto-oncogene, transcription factor
Database Link	Entrez Gene 17863 MouseEntrez Gene 4602 Human UniProt ID P10242
Background	Myb is a transcriptional activator; DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'. It participates in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells. TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation. A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation. Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.
Synonyms	c-Myb
Note	Calculated Molecular Weight: 85.5kDa according to NP_001123645.1).
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transcription Factors

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.