p27 KIP 1 (CDKN1B) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1/500-1/1000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of p27 protein.
Formulation	Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2 State: Aff - Purified State: Liquid purified Ig fraction
Concentration	1.0 mg/ml
Purification	Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 27 kDa
Gene Name	cyclin-dependent kinase inhibitor 1B
Database Link	Entrez Gene 1027 Human UniProt ID P46527
Background	Cell cycle progression is regulated by a series of cyclin-dependent kinases that consist of catalytic subunits, designated Cdks, and activating subunits designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has been recently described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner, p16 and a more recently described p16 related inhibitor designated p15. A p21 related protein, p27, has been described as a negative regulator of G1 progression and has been speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and to a lesser extent with cyclin E and Cdk2.
Synonyms	p27Kip1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer

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