Thermonuclease Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Recommended Dilutions: Non-precipitating antibody-binding techniques: 1/1,000-1/10,000.
Reactivity	Staphylococcus aureus
Host	Rabbit
Clonality	Polyclonal
Immunogen	Nuclease isolated and purified from Staphylococcus aureus. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	This antibody is a polyclonal immunologic reagent to Nuclease from Staphylococcus aureus. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Isotype	IgG
Formulation	PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized purified hyperimmune IgG fraction
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P00644
Background	Native Stapylococcus aureus Nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3'-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems.
Synonyms	TNase, Nuclease, nuc
Reference Data

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