Angpt2 (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect ANG2 in cells and tissues. Immunohistochemistry on Paraffin Sections: At 1-2μg/ml to detect ANG2 in formalin fixed and paraffin embedded.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence mapping near the C-terminal (454-468) of Human Angiopoietin-2.
Specificity	This antibody detects Angiopoietin-2 near C-term. No cross reactivity with other proteins.
Isotype	IgG
Formulation	0.9mg NaCl, 0.2mg Na2HPO4 State: Aff - Purified State: Lyophilized purified Ig fraction Stabilizer: 5mg BSA Preservative: 0.05mg Thimerosal, 0.05mg Sodium Azide
Reconstitution Method	0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	angiopoietin 2
Database Link	Entrez Gene 285 HumanEntrez Gene 89805 RatEntrez Gene 11601 Mouse UniProt ID O35608
Background	Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Synonyms	ANG-2, ANG2, ANGPT2
Reference Data

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