Il5 Rabbit Polyclonal Antibody

CAT#: AP23789BT-S

Il5 rabbit polyclonal antibody, Biotin

Size: 25 ug 50 ug

Conjugation: Unconjugated Biotin



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CNY 3,910.00


货期*
5周

规格
    • 25 ug

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Sandwich ELISA: To detect Mouse IL-5 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Mouse IL5 (AP23789PU-N or AP23789PU-S) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IL-5.
Direct ELISA: To detect Mouse IL-5 by Direct ELISA (using 100μl/well antibody solution) a concentration of ~1.0 μg/ml of this antibody is required. This Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IL-5.
Western Blot: To detect Mouse Il-5by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure recombinant Murine IL-5
Specificity Recognizes Interleukin-5/IL5.
Formulation PBS, pH 7.2 without preservatives
Label: Biotin
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing an immobilized Murine IL-5 matrix
Conjugation Biotin
Storage Condition Store the lyophilized antibody at -20°C.
Following reconstitution it is stable for two weeks at 2-8°C.
Frozen aliquots are stable for 6 months when stored at -20°C.
Avoid repeated freezing and thawing.
Gene Name interleukin 5
Background Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and Mouse IL5 have 70% amino acid sequence homology.
Synonyms IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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