ICAM1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Immunohistochemistry on frozen sections. Immunoprecipitation. Western blot. Immuhnoassay. The typical starting working dilution for Immunhistology and Western blot is 1:10. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 µg/ml) in tissue culture medium at 37°C.
Reactivity	Human, Monkey
Host	Rabbit
Clonality	Polyclonal
Specificity	The antibody reacts with both intact human ICAM-1, CD54 and with soluble human ICAM-1.
Isotype	IgG
Formulation	PBS Label: Biotin State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein A
Conjugation	Biotin
Storage Condition	Store at 2 - 8 °C.
Gene Name	intercellular adhesion molecule 1
Database Link	Entrez Gene 3383 Human UniProt ID P05362
Background	ICAM-1 (intercellular adhesion molecule) has 7 potential N-linked glycosylation sites. It is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1).
Synonyms	ICAM-1
Reference Data

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