c Kit (KIT) pTyr719 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	ELISA: The antibody (approx. 1:64,000 diluted) can detect the above Tyr 719 phosphorylated c-kit peptide coated on ELISA plate.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide derived from tyrosine 719 phosphorylated c-kit, conjugated with KLH
Specificity	This antibody reacts with Mouse, Human and other species with consensus c-kit peptide (SDSTNEYpMDMKPG).
Formulation	0.01M PBS, pH 7.4 State: Azide Free State: Lyophilized Ig fraction
Reconstitution Method	Restore with Double distilled water to adjust the final concentration to 1.00mg/ml.
Purification	Protein G affinity
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	KIT proto-oncogene receptor tyrosine kinase
Database Link	Entrez Gene 3815 Human UniProt ID P10721
Background	c-kit, also known as CD117, is a receptor tyrosine kinase expressed at the surface of mast cells and certain types of stem cells. c-kit is activated and autophosphorylated upon binding with stem cell factor. Activated c-kit phosphorylates downstream SH2- domain-containing signalling molecules such as PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL. The binding is impaired when c-kit is mutated at tyrosine719. c-kit with phosphorylated tyrosine 719 binds to the p85 subunit of PI3 kinase in vitro and in vivo. C-kit is encoded by KIT gene and the gene is frequently over-expressed in gastrointestinal stromal tumor, myelogenous leukemia, and testicular germ cell tumor. Mutational loci of the gene are implicated with responsiveness to cancer drug imatinib.
Synonyms	SCFR, KIT
Reference Data

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