NFATC4 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/16000 (Detection Limit). Western blot: No signal obtained yet but low background observed in MOLT-4, Jurkat, A431, HeLa, Human Brain and Human Heart lysates at upto 2 µg/ml. Immunohistochemistry on Paraffin Sections: 2.5-3.8 µg/ml. In paraffin embedded Human Placenta shows weak nuclear staining in trophoblasts.
Reactivity	Canine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C Terminus of the protein sequence according to NP_004545.2, NP_001185894.1, NP_001185895.1, NP_001185896.1.
Specificity	This antibody is expected to recognise isoform 2 (NP_004545.2), Isoform 3 (NP_001185894.1), Isofrom 4 (NP_001185895.1) and Isofrom 5 (NP_001185896.1).
Formulation	Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	nuclear factor of activated T-cells 4
Database Link	Entrez Gene 73181 MouseEntrez Gene 305897 RatEntrez Gene 4776 Human UniProt ID Q14934
Background	The protein is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a pre-existing cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4.
Synonyms	NF-ATc4
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway

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