APOBEC3A_B Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	Peptide ELISA: 1/8000 (Detection limit). Western blot: 3-6 µg/ml. Approx. 26-28kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0kDa according to Human NP_663745.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature. Immunohistochemitry on Paraffin Sections: 3-6 µg/ml. In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the nucleus of A431 and A549 cells. Flow Cytometry: 10 µg/ml. Flow cytometric analysis of Jurkat cells.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide with sequence from the internal region of Human APOBEC3A (NP_663745.1).
Specificity	This antibody recognizes Human Phorbolin 1 / APOBEC3A. Other species not tested.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	APOBEC3A and APOBEC3B deletion hybrid
Database Link	Entrez Gene 100913187 Human UniProt ID P31941
Background	PHO1 a member of the cytidine deaminase gene family. The PHO1 gene is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. This gene encodes a protein that lacks the zinc binding activity and may be an expressed pseudogene.
Synonyms	Phorbolin-1
Reference Data

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