Apolipoprotein A IV / ApoA4 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit) Western blot: Preliminary experiments in Mouse and Rat Liver lysates gave no specific signal but low background (at antibody concentration up to 1 µg/ml). Immunohistochemistry on Paraffin Sections: 2-4 µg/ml. In paraffin embedded Mouse Colon shows staining of cells between the villi, consistent with the observations with the related AP16668PU-N.
Reactivity	Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_031494.2.
Specificity	Recognizes Mouse Aolipoprotein A-IV.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	apolipoprotein A-IV
Database Link	Entrez Gene 11808 Mouse UniProt ID P06728
Background	APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In Rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties.
Synonyms	Apolipoprotein A-IV, ApoA-IV, Apo-AIV
Reference Data

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