HIC1 (193-204) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: 1/128000 (Detection Limit). Western blot: 0.5-1 μg/ml. In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2.
Reactivity	Canine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_006488.2; NP_001091672.1.
Specificity	This antibody is expected to recognize both reported isoforms (NP_006488.2; NP_001091672.1).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	hypermethylated in cancer 1
Database Link	Entrez Gene 3090 Human UniProt ID Q14526
Synonyms	HIC-1
Reference Data

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