GDNF Receptor alpha 1 (GFRA1) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, WB
Recommend Dilution	ELISA: 1/80000-1/160000. Western blot: 1/5000-1/10000. Immunofluorescence: 1/1000-1/2000.
Reactivity	Human
Host	Chicken
Clonality	Polyclonal
Immunogen	Recombinant His-tagged human GFRa-1 protein produced using CHO-based. For production of hGFRa-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Specificity	This antibody detects Human GFRa-1 and doesn’t cross-react with hGFRa-2, hGFRa-3, hGFRa-4. Other species not tested.
Isotype	IgY
Formulation	Concentrated Ammonium Sulphate in PBS pH 7.4 State: Aff - Purified State: Liquid purified IgY fraction
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store at 2-8°C. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use.
Gene Name	GDNF family receptor alpha 1
Database Link	Entrez Gene 2674 Human UniProt ID P56159
Background	Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFRa-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).
Synonyms	GFR-alpha-1, GDNF receptor alpha, RET ligand 1, GDNFRA, RETL1, TRNR1
Reference Data

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